Tm
Algorithm
Bio.SeqUtils.MeltingTemp.Tm_NN
nn_table
DNA_NN1 - Breslauer et al. (1986), Proc Natl Acad Sci USA 83: 3746-3750
DNA_NN2 - Sugimoto et al. (1996), Nuc Acids Res 24 : 4501-4505
DNA_NN3 - Allawi and SantaLucia (1997), Biochemistry 36: 10581-10594
DNA_NN4 - SantaLucia & Hicks (2004), Annu. Rev. Biophys. Biomol. Struct 33: 415-440
saltcorr
1. 16.6 x log[Na+] (Schildkraut & Lifson (1965), Biopolymers 3: 195-208)
2. 16.6 x log([Na+]/(1.0 + 0.7*[Na+])) (Wetmur (1991), Crit Rev Biochem Mol Biol 126: 227-259)
3. 12.5 x log(Na+] (SantaLucia et al. (1996), Biochemistry 35: 3555-3562
4. 11.7 x log[Na+] (SantaLucia (1998), Proc Natl Acad Sci USA 95: 1460-1465
5. Correction for deltaS: 0.368 x (N-1) x ln[Na+] (SantaLucia (1998), Proc Natl Acad Sci USA 95: 1460-1465)
6. (4.29(%GC)-3.95)x1e-5 x ln[Na+] + 9.40e-6 x ln[Na+]^2 (Owczarzy et al. (2004), Biochemistry 43: 3537-3554)
7. Complex formula with decision tree and 7 empirical constants.
Na
mM
Tris
mM
Mg
mM
K
mM
dnac1
µM
dNTPs
mM
dnac2
µM
>MyPrimer ATGGCAGTTGAGAAGA >another agtgtgctagtagtacgtcgta
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