WebPCR simulator | Simulate PCR if you already have a pair of primers and a template sequence. |
Primer designer | Design a new primer pair for a template sequence or list of template sequences. |
Matching primer | Design a primer to match an existing primer for a primer/template pair or list of such pairs. |
Assembly designer | Design primers for linear or circular assembly of a list of amplicons or sequence fragments. |
Assembly simulator | Simulator for linear or circular assembly of a list of sequence fragments. |
Primer Tm calculator | Melting temperature calculator for PCR primers. Uses the Bio.SeqUtils.MeltingTemp.Tm_NN function from Biopython. |
Restriction simulator | Simulate restriction digestion. |
CRISPr simulator | Simulate CRISPr digestion. |
Toggle format | Reformat sequences in GenBank or FASTA format. |
Repair GenBank | Repair malformed GenBank files. |
Gateway | Simulate Gateway cloning. |
SEGUID | Calculate SEGUID checksums for protein or DNA sequences. |
Fusion PCR | Simulate fusion PCR. |
Primer enumerator | Assign numbers to primers in a list (FASTA or GenBank format). Nunmber will be prepended to identifier. |
Format primer list in TAB format | Format primer list in TAB format. Required for many companies selling oligonucleotides. |
Documentation | Documentation and examples. |