PydnaWeb PydnaWeb powered by pydna 6.0.0-a.30 with Biopython 1.83
WebPCR simulator Simulate PCR if you already have a pair of primers and a template sequence.
Primer designer Design a new primer pair for a template sequence or list of template sequences.
Matching primer Design a primer to match an existing primer for a primer/template pair or list of such pairs.
Assembly designer Design primers for linear or circular assembly of a list of amplicons or sequence fragments.
Assembly simulator Simulator for linear or circular assembly of a list of sequence fragments.
Primer Tm calculator Melting temperature calculator for PCR primers. Uses the Bio.SeqUtils.MeltingTemp.Tm_NN function from Biopython.
Restriction simulator Simulate restriction digestion.
CRISPr simulator Simulate CRISPr digestion.
Toggle format Reformat sequences in GenBank or FASTA format.
Repair GenBank Repair malformed GenBank files.
Gateway

Simulate Gateway cloning.

SEGUID

Calculate SEGUID checksums for protein or DNA sequences.

Fusion PCR Simulate fusion PCR.
Primer enumerator Assign numbers to primers in a list (FASTA or GenBank format). Nunmber will be prepended to identifier.
Format primer list in TAB format Format primer list in TAB format. Required for many companies selling oligonucleotides.
Documentation Documentation and examples.